variant-aware-cas-offinder-v2

Variant-aware Cas-OFFinder pipeline (version 2) that identifies potential CRISPR off-target sites accounting for individual genetic variants. The pipeline normalizes a phased single-sample VCF using vcfallelicprimitives, bcftools norm, and vcfcreatemulti, splits by chromosome to avoid segfaults, generates allelic FASTA files with vcf2fasta, and runs cas-offinder per individual FASTA file.

Based on: External workflow