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bulk-rnaseq-preprocessing

Bulk RNA-seq preprocessing pipeline that wraps nf-core/rnaseq (v3.22.2) with the STAR-Salmon workflow. Takes raw FASTQ files and a samplesheet as input, performs quality control, trimming, alignment with STAR, quantification with Salmon, and produces a comprehensive MultiQC report along with gene/transcript-level count matrices.

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VERSION 1.0.0
AUTHOR khyeonm
INPUT samplesheet.csvfastq.gzgenome.fagenes.gtf
OUTPUT multiqc_report.htmlsalmon.merged.gene_counts.tsvsorted.bam
TOOLS
Nextflownf-core/rnaseqSTARSalmon
TAGS
RNA-seqbulk RNA-seqpreprocessingnf-coregene expressiontranscriptomicsquantificationalignment
FILES

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v1.0.0 latest
2026-05-14 ยท khyeonm